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Stability of mercurated DNA during reassociation and S1 nuclease digestion experiments

โœ Scribed by Virginia Walbot


Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
464 KB
Volume
90
Category
Article
ISSN
0003-2697

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Use of S1 nuclease and formamide in comb
โœ Chanchal Sadhu; Santanu Datta; K.P. Gopinathan ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 403 KB

The optimal parameters in the use of nuclease S1 in DNA reassociation kinetics in the presence of formamide have been determined. The conditions are especially suitable for the study of DNA rich in mole percent GC. A 10-fold dilution of the reassociation samples leading to a decrease in both NaCl an