Use of S1 nuclease and formamide in comb
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Chanchal Sadhu; Santanu Datta; K.P. Gopinathan
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Article
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1984
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Elsevier Science
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English
โ 403 KB
The optimal parameters in the use of nuclease S1 in DNA reassociation kinetics in the presence of formamide have been determined. The conditions are especially suitable for the study of DNA rich in mole percent GC. A 10-fold dilution of the reassociation samples leading to a decrease in both NaCl an