Ca2+ Regulation in Differentiating Lens Cells in Culture
✍ Scribed by Grant C. Churchill†; Charles F. Louis*
- Book ID
- 115605886
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 583 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0014-4835
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Previous studies from our laboratory have led us to conclude that lens cell elongation is caused by an increase in cell volume. This volume increase results from an increase in the potassium content of the cells due to decreased potassium efflux. In contrast, an increase in the volume of most cells
During terminal differentiation of lens epithelial cells into fiber cells, nuclei become pycnotic and DNA degradation occurs. We investigated the putative role in this process of an endogenous DNAase. After incubation of isolated nuclei of both cell types at 37OC, DNAase activity was revealed by DNA
The intracellular pH (pHi) of tissue-cultured bovine lens epithelial cells was measured in small groups of 6 to 10 cells using the trapped fluorescent dye 2',7'-bis-(2-,carboxyethyl)-5 (and 6)carboxyfluorescein (BCECF). When perifused at 35 degrees C with artificial aqueous humour solution (AAH) con