c-jun expression and growth stimulation in human ovarian carcinoma cell lines following exposure to cytokines

✍ Scribed by Daniel M. Spinner; Thomas Brandstetter; Marion Kiechle-Schwarz; Andreas Du Bois; Peter Angel; Thomas Bauknecht

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: French
• Weight: 884 KB
• Volume: 63
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910630321

✦ Synopsis

We evaluated the effects of recombinant human G-CSF, rhGM-CSF. rhM-CSF and rhlL-I Q on proliferation and regulation of c-jun gene expression in 4 human ovarian-carcinoma (HOC) cell lines, NIH:OVCAR-3, SK-OV-3, HEY and BG-I, and in one primary ovarian tumor in vitro. The cytokines were administered in concentrations of 0. I U/ml to 1000 U/ml. Cell growth was measured by crystal-violet-and thiazolyl-blue(MlT)based cell counts. c-jun transcripts were measured by the solution hybridization/RNAse protection assay. RhM-CSF and rhGM-CSF showed no growth stimulation of any of the 5 cell lines tested. Results from exposure to rhG-CSF were different. The cell lines NIH:OVCAR-3, SK-OVJ, BG-I and the primary ovarian tumor showed no proliferative response. A 2to 3-fold increase in proliferation was observed in the HEY HOC cell line. rhlL-la led to growth stimulation in the BG-I cell line, but showed an inhibitory effect in the NIHOVCAR-3 cells. No effects of rhlL-I Q were observed in the remaining 2 cell lines nor in the primary ovarian tumor. Growth stimulation was accompanied by an increase in c-jun expression in the HEY cell line, and the BG-l cell line. No alterations in c-jun expression were observed in the remaining 3 cell lines. Our results indicate that rhG-CSF or rhlL-la influence cell proliferation in 2 out of 5 human ovarian-tumor cell lines, accompanied by an increase in c-jun expression.