✦ LIBER ✦

Butyrylcholinesterase in Human Brain and Acetylcholinesterase in Human Plasma: Trace Enzymes Measured by Two-Site Immunoassay

✍ Scribed by Stephen Brimijoin; Pamela Hammond

Book ID: 111174862
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 464 KB
Volume: 51
Category: Article
ISSN: 0022-3042
DOI: 10.1111/j.1471-4159.1988.tb03091.x

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Novel and sensitive noncompetitive enzym

Novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for α-human atrial natriuretic peptide in plasma

✍ Seiichi Hashida; Naoko Yamamoto; Eiji Ishikawa 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 585 KB

A novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for a-human atrial natriuretic peptide (a-hANP) in plasma, which uses only one monoclonal IgG for the ring structure of a-hANP, is described. Plasma was filtered through polysaccharide membrane to separate p

Direct measurement of α-human atrial nat

Direct measurement of α-human atrial natriuretic polypeptide in plasma by sensitive enzyme immunoassay

✍ Seiichi Hashida; Eiji Ishikawa; Masashi Mukoyama; Kazuwa Nakao; Hiroo Imura 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 634 KB

a-Human atrial natriuretic polypeptide (a-was 30-90 fg (10-30 attomo1es)ltube and hANP) in plasma was directly measured 0.6-2.3 ng (0.2-0.75 pmol)/L using 0.04without extraction by sensitive sandwich 0.05 ml of plasma. The lower detection enzyme immunoassay. Polystyrene balls limit was obtained usin

Reassessment of the two-site enzyme immu

Reassessment of the two-site enzyme immunoassay for human epidermal growth factor (hegf) and measurement of immunoreactive hegf in human sera and urine

✍ Yoshifumi Takei; Hisayo Ezawa; Kyozo Hayashi; Masayuki Kurobe 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 507 KB

We reassessed the enzyme immunoassay (EIA) system for hEGF previously developed by our laboratory ( C h ChimActa 15651-60, 1985), since it appeared that the reported EIA system detected not only hEGF but also pS2 protein owing to minor contamination by pS2 protein in the hEGF sample used for immuniz

A sandwich enzyme immunoassay for detect

A sandwich enzyme immunoassay for detecting and measuring alzheimer's disease-associated proteins in human brain tissue

✍ Hossein A Ghanbari; Hossein A Ghanbari; Terry Kozuk; Barney E. Miller; Sue Riesi 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 274 KB

## Abstract A simple, fast, and reliable immunoassay has been developed to detect and measure Alzheimer's disease‐associated proteins (ADAPs) in human brain tissue. This assay, called ALZ‐EIA (brain), was developed for Abbott's quantum system, in which 1/4‐in. beads are used as solid phase. The bea

Noncompetitive enzyme immunoassay (heter

Noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for γ-melanocyte-stimulating hormone (γ-msh) and measurement of immunoreactive γ-msh in plasma of healthy subjects

✍ Yoshikuni Yogi; Seiichi Hashida; Rolf Ekman; Toshiaki Setoguchi; Dr. Eiji Ishika 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 832 KB

A noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for p-melanocyte-stimulating hormone (@-MSH) was developed. p-MSH (1-12) was biotinylated, trapped onto an anti-e-MSH (1-12) IgG-coated polystyrene bead, eluted at pH 1 after washing to eliminate other biotinylated substances,

Development of a highly sensitive and sp

Development of a highly sensitive and specific two-site enzyme immunoassay for parathyroid hormone (1-34): Application to pharmacokinetic study on intranasal parathyroid hormone (1-34) in human

✍ Takeyuki Kohno; Noriaki Murasugi; Hiroki Sakurai; Kazuhito Watabe; Hiromichi Nak 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 86 KB 👁 2 views

A highly sensitive and specific two-site enzyme immunoassay for parathyroid hormone (1-34) (PTH(1-34)) and its usability for the pharmacokinetic study are described. Plasma samples were incubated simultaneously with 2,4-dinitrophenylated anti-PTH(1-34) IgG and anti-PTH(1-34) Fab´β-D-galactosidase co