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Buffer effect on the kinetics of ornithine carbamyl transferase by hplc

✍ Scribed by V. Carunchio; A. M. Girelli; A. Messina

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
75 KB
Volume
13
Category
Article
ISSN
0269-3879

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✦ Synopsis

A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the direct determination of citrulline. A different kinetic pattern was observed in tris(hydroxymethyl)aminomethane (TRIS) and triethanolamine (TEA) buffers: a ping-pong or an ordered sequential mechanism are suggested, respectively. The inhibition of Tris buffer on OCT reaction has also been demonstrated. The inhibition of Tris buffer on OCT reaction has also been demonstrated.

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