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BsuCI, a type-I restriction-modification system in bacillus subtilis

✍ Scribed by Guoliang Xu; Jürgen Willert; Wolfgang Kapfer; Thomas A. Trautner

Book ID: 116133271
Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 75 KB
Volume: 157
Category: Article
ISSN: 0378-1119
DOI: 10.1016/0378-1119(95)00724-k

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Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h