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Cloning and expression of the BamHI restriction-modification system in Bacillus subtilis

โœ Scribed by Bunsei Kawakami; Nobuhiro Katsuragi; Yoshihiko Maekawa; Tadayuki Imanaka

Book ID
113396248
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
381 KB
Volume
70
Category
Article
ISSN
0922-338X

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Restriction and modification in Bacillus
Restriction and modification in Bacillus subtilis: Expression of the cloned methyltransferase gene from B. subtilis phage SPR in E. coli and B. subtilis
โœ Montenegro, M. A. ;Pawlek, B. ;Behrens, B. ;Trautner, T. A. ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer ๐ŸŒ English โš– 451 KB

Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h