Breakpoint clusters of the PML gene in acute promyelocytic leukemia: Primary structure of the reciprocal products of the PML-RARA gene in a patient with t(15;17)

✍ Scribed by Shuo Dong; Jie-Ping Geng; Jia-Hua Tong; Yu Wu; Jin-Ren Cai; Guan-Lin Sun; Shu-Rong Chen; Zhen-Yi Wang; Christian-Jacques Larsen; Roland Berger; Sai-Juan Chen; Zhu Chen

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 623 KB
• Volume: 6
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870060302

✦ Synopsis

D N A studies of the translocation t( 15; 17) in acute promyelocytic leukemia (APL) have shown that the retinoic acid receptor alpha (RARA) gene on chromosorne I7 is juxtaposed to the promyelocytic leukemia (PML) gene on chromosome 15. The PML breakpoints have been mapped to1 3 clusters: bcr I, bcr2, and bcr3. W e have examined the PML breakpoint distribution in a series of 33 Chinese patients with APL. Twenty-two patients fell within bcr I, 2 within bcr2, and 9 within bcr3. The primary structure of the reciprocal chromosome translocation joints of one patient and that of their normal counterparts have been determined and compared to those of 2 previiously reported cases. These studies revealed possible topoisomerase II cleavage sites close to the breakpoints and suggested implications of D N A attachment sites to nuclear matrix. W e propose that these features are relevant to the process of illegitimate recombination generating the translocation. Genes Chrom Concer 6: 133-139 ( / 993).