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Branched-chain amino acid aminotransferase activity in the tissues of lake trout

โœ Scribed by Hughes, Steven G.; Rumsey, Gary L.; Nesheim, Malden C.

Book ID
123477653
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
235 KB
Volume
76
Category
Article
ISSN
1096-4959

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A method for branched-chain amino acid aminotransferase is described which is based on running the reaction in the reverse of the usual direction with glutamate and r\*-ketoisocaproate as substrates. The cY-ketoglutarate generated is reduced with glutamate dehydrogenase and NADH. For sensitivity in