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Branched chain 2-oxo-acid dehydrogenase complex of rat liver

โœ Scribed by Peter J. Parker; Philip J. Randle


Book ID
115908503
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
180 KB
Volume
90
Category
Article
ISSN
0014-5793

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Purification of branched chain ฮฑ-ketoaci
โœ Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 452 KB

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s