## Abstract Brain derived neurotrophic factor (BDNF) exhibits a sequence of actions on neurons ranging from acute enhancement of transmission to longβterm promotion of neurite outgrowth and synaptogenesis associated with learning and memory. The manifold effects of BDNF on neuronal modifications ma
Brain-derived neurotrophic factor stimulates neurite outgrowth in a calretinin-enriched neuronal culture system.
β Scribed by Katsunori Iwasaki; Krystyna r Isaacs; David M Jacobowitz
- Book ID
- 117563843
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 998 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0736-5748
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## Abstract To visualize the release dynamics of the brainβderived neurotrophic factor (BDNF) involved in neural plasticity, we constructed a plasmid encoding green fluorescent protein (GFP) fused with BDNF. First, several biological studies confirmed that this fusion protein (BDNFβGFP) mimics the
We used compartmented cultures into NGF plus NT3 compartments, while the combinato study the regulation of adult sensory neurite growth tion of BDNF plus NGF resulted in an inhibition of by neurotrophins. We examined the effects of the neuneurite extension compared with NGF alone. We then rotrophins