Bovine aortic endothelial cells release hydrogen peroxide

✍ Scribed by T. Sundqvist

• Book ID: 102887020
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 502 KB
• Volume: 148
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041480118

✦ Synopsis

Endothelial cells grown on microcarriers are able to release H,O, to the extracellular environment without any added stimulus. The extracellularly released H,O, can be detected by luminol-amplified chemiluminescence (CL) if horseradish peroxidase is added. The CL response can be reduced by catalase and blocked by superoxide dismutase, indicating that 0,could be a precursor for H,O,. The CL kinetics, i.e., a long lag time followed by a rapid shift to a new level, indicate activation of an 0,--producing enzyme. The cells are also able to protect themselves from H,O, stimulation by both catalase and the glutatione system. Bradykinin stimulates the H,O, release, but if the effect is directly stiniulatory or if it acts by reduction of the protective system is at present unclear. The extracellularly released H, O, could be a cause of injury to the endothelial cells or to the subendothelial matrix.