Toxic effects of aldehydes released from fixed pericardium on bovine aortic endothelial cells

✍ Scribed by Eybl, E. ;Griesmacher, A. ;Grimm, M. ;Wolner, E.

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 571 KB
• Volume: 23
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820231111

✦ Synopsis

Glutaraldehyde (GA) and formaldehyde (FA) were shown to be released from 1 cm2 fixed pericard patches into 2-mL storage solutions after three 2-min washings in concentrations of about 1 mg/L. The cytotoxicity of these aldehyde concentrations on bovine aortic endothelial cells (BAECs) was evaluated in vitro, by proliferation capacity, cellular ATP content, PG12 release and cyclic AMP synthesis. Continuous incubation of BAECs with GA > 0.1 mg/L and FA > 0.5 mg/L resulted in a signifi-cantly inhibited proliferation and in an increase of the intracellular Ca2+ triggered parameters, PG12 and cyclic AMP, up to three fold. This strongly suggests an aldehyde-induced inhibition of the plasmamembrane bound Ca2*-ATPase, an enzyme which normally maintains low intracellular Ca2+-level. From these findings there is evidence that aldehydes released from bioprosthetic valve tissue may contribute to the lack of endothelial cell coverage in human implants.