Boosting the Resolution of 1 H NMR Spectra by Homonuclear Broadband Decoupling
β Scribed by Meyer, N. Helge; Zangger, Klaus
- Book ID
- 124064455
- Publisher
- John Wiley and Sons
- Year
- 2013
- Tongue
- English
- Weight
- 731 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1439-4235
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## Abstract Twoβdimensional ^1^H NMR experiments that achieve bandβselective, homonuclear decoupling in both the indirectly detected F1 and directly detected F2 dimensions were used to assign the highly overlapped ^1^H NMR spectrum of the peptide AcβSRGKARVRAKVKDQTKβNH~2~, both free in solution and
Application of an decoupled ROESY (BASHD-ROESY) experi-u 1 -band-selective, u 1 -homonuclear ment to the assignment of 1H NMR spectra of peptides is demonstrated. Band selection in the dimension is u 1 achieved with the double pulsed Γeld gradient spin echo (DPFGSE) technique ; homonuclear decouplin