Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold

✍ Scribed by Hidemi Hattori; Kazunori Masuoka; Masato Sato; Miya Ishihara; Takashi Asazuma; Bonpei Takase; Makoto Kikuchi; Koichi Nemoto; Masayuki Ishihara

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 768 KB
• Volume: 76B
• Category: Article
• ISSN: 1552-4973
• DOI: 10.1002/jbm.b.30357

✦ Synopsis

Abstract

Human adipose tissue, obtained by liposuction, was processed to obtain a fibroblast‐like population of cells or adipose tissue‐derived stromal cells (ATSCs). The ATSCs, as well as bone marrow‐derived mesenchymal stem cells (BMSCs), have the capacity for renewal and the potential to differentiate into multiple lineages of mesenchymal tissues. These cells are capable of forming bone when implanted ectopically in an appropriate scaffold. The aim of this study was to evaluate a β‐tricalcium phosphate (β‐TCP) as a scaffold and to compare the potential of osteogenic differentiation of ATSCs with BMSCs. Both cell types were loaded into β‐TCP disk and cultured in an osteogenic induction medium. Optimal osteogenic differentiation in ATSCs in vitro, as determined by secretion of osteocalcin, scanning electron microscope, and histology, were obtained in the culturing with the β‐TCP disk. Furthermore, bone formation in vivo was examined by using the ATSC‐ or BMSC‐loaded scaffolds in nude mice. The present results show that ATSCs have a similar ability to differentiate into osteoblasts and to synthesize bone in β‐TCP disk as have BMSCs. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006