Meniscal regeneration using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow
✍ Scribed by Takuma Yamasaki; Masataka Deie; Rikuo Shinomiya; Yasunori Izuta; Yuji Yasunaga; Shinobu Yanada; Patrick Sharman; Mitsuo Ochi
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 419 KB
- Volume
- 75A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
The purpose of this study was to regenerate a meniscus using a scaffold from a normal meniscus and mesenchymal stromal cells derived from bone marrow (BM‐MSCs). Thirty Sprague‐Dawley rat menisci were excised and freeze‐thawed three times with liquid nitrogen to kill the original meniscal cells. Bone marrow was aspirated from enhanced green fluorescent protein transgenic Sprague‐Dawley rats. BM‐MSCs were isolated, cultured for 2 weeks, and 2 × 10^5^ cells were then seeded onto the meniscal scaffolds. Using a fluorescent microscope and immunohistochemical staining, repopulation of enhanced green fluorescent protein positive cells was observed in the superficial zone of the scaffold after 1 week of culture, and then in the deep zone after 2 weeks. At 4 weeks, expression of extracellular matrices was detected histologically and expression of mRNA for aggrecan and type X collagen was detected. Stiffness of the cultured tissue, assessed by the indentation stiffness test, had increased significantly after 2 weeks in culture, and approximated the stiffness of a normal meniscus. From this study, we conclude that a scaffold derived from a normal meniscus seeded with BM‐MSCs can form a meniscus approximating a normal meniscus. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005