Bleomycin resistance as a selectable marker for transformation of the eukaryote, Dictyostelium discoideum
β Scribed by Chang, Andy C.M.; Hall, Ruth M.; Williams, Keith L.
- Book ID
- 123510178
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 717 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0378-1119
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A plasmid (pAmN61) containing the NPT II structural gene (neomycin phosphotransferase) fused to the N-terminal region of a homologous actin gene was used for the transformation of Absidia glauca protoplasts. Neomycin resistant transformants could be selected for on complete medium containing 1.2 mg/