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Neomycin resistance as a dominantly selectable marker for transformation of the zygomyceteAbsidia glauca

✍ Scribed by Johannes Wöstemeyer; Anke Burmester; Christoph Weigel

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
342 KB
Volume
12
Category
Article
ISSN
0172-8083

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✦ Synopsis

A plasmid (pAmN61) containing the NPT II structural gene (neomycin phosphotransferase) fused to the N-terminal region of a homologous actin gene was used for the transformation of Absidia glauca protoplasts. Neomycin resistant transformants could be selected for on complete medium containing 1.2 mg/ml neomycin sulfate. The physical presence of plasmid DNA in Absiclia glauca was demonstrated by retransformation into Escherichia coli and by Southern blot analysis. No integration of plasmid DNA at either one of the two actin loci was observed; Southern blot experiments provide evidence that pAmN61 is autonomously replicated in Absidia glauca.

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