Biphasic response to human galanin of extracellular acidification in human Bowes melanoma cells
β Scribed by Roland Lang; Alexandra Berger; Anton Hermann; Barbara Kofler
- Book ID
- 114014068
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 217 KB
- Volume
- 423
- Category
- Article
- ISSN
- 0014-2999
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A comparison of the production of tissue-type plasminogen activator (t-PA) and gelatinases A and B was made at the mRNA and protein levels in human Bowes melanoma cells treated with phorbol myristate acetate (PMA). Immunocytochemical analysis confirmed previous quantitative data on PMA-mediated indu
Bowes melanoma cells were cultivated successfully in a serum-free medium which was constructed by the concept of maximum retention of proteins from fractionated human plasma having growth stimulatory activities. The cells could be cultivated in the serurn-free medium without any adaptation period. T