Microcarrier culture of bowes melanoma cells in serum-free medium with Human plasma fraction IV-4+ V
✍ Scribed by Eui-Cheol Jo; Man Bock Gu; Dong-II Kim; Sung Bok Paik
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 574 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
Bowes melanoma cells were cultivated successfully in a serum-free medium which was constructed by the concept of maximum retention of proteins from fractionated human plasma having growth stimulatory activities. The cells could be cultivated in the serurn-free medium without any adaptation period. The major serum-free component of the medium was the fraction IV-4+V of the Cohn fractionation process of human plasma. Approximately six times increase of tissue-type plasminogen activator (t-PA) activity as compared with that in serum-supplemented medium was observed in this serum-free medium even though the cell growth was much slower. In addition, the growth stimulatory activities of thrombin and fibronectin were investigated during the cultivation of Bowes melanoma cells in this serurn-free medium. These proteins contributed significantly to the enhanced growth of cells by reducing doubling time to 25 and 35 h as compared with 55 h in the serumfree medium without them. Especially, fibronectin sup- ported cells to propagate near to the maximum cell density achieved in the medium with 10% FBS.