𝔖 Bobbio Scriptorium
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Biotinylated proteins as molecular weight standards on Western blots

✍ Scribed by Dean Della-Penna; Rolf E. Christoffersen; Alan B. Bennett

Book ID
102986242
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
548 KB
Volume
152
Category
Article
ISSN
0003-2697

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✦ Synopsis

Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-poiyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase complex and reaction with 4chloro-l-naphthol and hydrogen peroxide. The biotinylated proteins are easy to prepare and are useful as molecular weight standards with most procedures employing immunodetection of proteins following transfer to nitrocellulose. paper.

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