Calibration of prestained protein molecular weight standards for use in the “Western” or enzyme-linked immunoelectrotransfer blot techniques

✍ Scribed by Victor C.W. Tsang; Kathy Hancock; A.Ray Simons

• Book ID: 102987178
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 845 KB
• Volume: 143
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90667-5

✦ Synopsis

Prestained protein molecular weight standards allow easy, direct visual location of electrophoretically transblotted lanes on nitrocellulose. They also provide a simple and accurate means for calibrating the molecular weights of resolved bands. Commercial prestained protein molecular weight standards, however, appear to have significantly different molecular weights from the original unstained proteins. We describe a calibration of these prestained molecular weight standards.