Biotechnology for the production of nutraceuticals enriched in conjugated linoleic acid: II. Multiresponse kinetics of the hydrolysis of corn oil by a Pseudomonas sp. lipase immobilized in a hollow-fiber reactor

✍ Scribed by Prima S. Sehanputri; Charles G. Hill Jr.

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 118 KB
• Volume: 69
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/1097-0290(20000820)69:4<450::aid-bit11>3.0.co;2-v

✦ Synopsis

The hydrolysis of corn oil in the presence of a lipase from Pseudomonas sp. immobilized within the walls of a hollow-fiber reactor was studied at 30°C. To assess the selectivity of this immobilized enzyme, the effluent concentrations of five different free fatty acids were measured using high-performance liquid chromatography (HPLC). Several rate expressions associated with a generic ping-pong bi-bi mechanism were used to fit the experimental data for this lipase-catalyzed reaction. A multiresponse nonlinear regression method was employed to determine the kinetic parameters associated with these rate expressions. Quasi-optimum operating conditions corresponded to 30°C and a buffer pH value of 7.0. Under these conditions, the concentration of free linoleic acid (C18:2) (the fatty acid of primary interest) in the effluent oil stream for a fluid residence time of 6 h was approximately 0.5 M.