Biotechnology for the production of nutraceuticals enriched in conjugated linoleic acid: I. Uniresponse kinetics of the hydrolysis of corn oil by a Pseudomonas sp. lipase immobilized in a hollow fiber reactor

✍ Scribed by Prima S. Sehanputri; Charles G. Hill Jr.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 227 KB
• Volume: 64
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19990905)64:5<568::aid-bit7>3.0.co;2-e

✦ Synopsis

The kinetics of the hydrolysis of corn oil in the presence of a lipase from Pseudomonas sp. immobilized within the walls of a hollow fiber reactor can be modeled in terms of a three-parameter rate expression. This rate expression consists of the product of a two-parameter rate expression for the hydrolysis reaction itself (which is of the general Michaelis-Menten form) and a first-order rate expression for deactivation of the enzyme.

Optimum operating conditions correspond to 30°C and buffer pH values of 7.0 during both immobilization of the enzyme and the hydrolysis reaction. Under these conditions, the total fatty acid concentration in the effluent oil stream for a fluid residence time of 4 h is approximately 1.6 M. This concentration corresponds to hydrolysis of approximately 50% of the glyceride bonds present in the feedstock corn oil. The fatty acid of primary interest in the effluent stream is linoleic acid.