Bioprocessing: an exciting new engineering discipline. It combines the development and optimization of biotechnological processes with effective strategies to recover and purify the desired products. Safety as well as cost play an important role here.
This volume covers the immensely differentiated spectrum of techniques and operations of bioprocessing, presented by the most competent experts in the field. An overview of upstream and downstream processing is given, fermentation and cell culture processes and the design of microbial fermenters are presented. A closing group of chapters is dedicated to issues of process validation, measurement, and regulation.
Topics included are: Industrial Cell Cultures/ Pharmaceutical Proteins/ Bioreactors/ Media and Air Sterilization/ Oxygen Transfer/ Scale Implications/ Fermentation Data Analysis/ Cell and Debris Removal/ Protein Purification/ Electrokinetic Separations/ Final Recovery Steps/ Process Validation
Content:
Chapter 1 Fermentation: An Overview (pages 7โ22): Barry C. Buckland and Malcolm D. Lilly
Chapter 2 Industrial Animal Cell Culture (pages 23โ38): Brian D. Kelley, Tzyy?Wen Chiou, Morris Rosenberg and Daniel I. C. Wang
Chapter 3 Overview of Downstream Processing (pages 39โ55): Ron Spears
Chapter 4 Proteins and Peptides as Drugs: Sources and Methods of Purification (pages 57โ73): Stuart E. Builder, Robert L. Garnick, James C. Hodgdon and John R. Ogez
Chapter 5 Bioreactors: Description and Modelling (pages 77โ104): Jens Nielsen and John Villadsen
Chapter 6 Cell Culture Bioreactors (pages 105โ126): Athanassios Sambanis and Wei?Shou Hu
Chapter 7 Media for Microbial Fermentations (pages 127โ139): Randolph L. Greasham
Chapter 8 Media for Cell Culture (pages 141โ156): R. A. Wove
Chapter 9 Media and Air Sterilization (pages 157โ184): Gokaraju K. Raju and Charles L. Cooney
Chapter 10 Oxygen Transfer and Mixing: Scale?Up Implications (pages 185โ217): Matthias Reuss
Chapter 11 Aeration in Cell Culture Bioreactors (pages 219โ281): John G. Aunins and Hans?Jurgen Henzler
Chapter 12 Strategies for Fermentation with Recombinant Organisms (pages 283โ294): Tadayuki Imanaka
Chapter 13 Anaerobic Fermentations (pages 295โ318): Larry E. Erickson, Daniel Y. C. Fung and Pravate Tuitemwong
Chapter 14 Fermentation Monitoring and Control (pages 319โ354): Thomas Chattaway, Gary A. Montague and A. Julian Morris
Chapter 15 Fermentation Data Analysis for Diagnosis and Control (pages 355โ400): Gregory Stephanopoulos, Konstantin Konstantinov, Urs Saner and Toshiomi Yoshida
Chapter 16 Design of Aseptic, Aerated Fermentors (pages 401โ426): Marvin Charles and Jack Wilson
Chapter 17 Biotransformations and Enzyme Reactors (pages 427โ466): Andreas S. Bommarius
Chapter 18 Cell and Cell Debris Removal: Centrif Ugation and Crossflow Filtration (pages 469โ503): Rajiv V. Datar and Carl?Gustaf Rosen
Chapter 19 Cell Disruption and Isolation of Non?Secreted Products (pages 505โ526): Horst Schutte and Maria?Regina Kula
Chapter 20 In vitro Protein Refolding (pages 527โ555): Jeffrey L. Cleland and Daniel I. C. Wang
Chapter 21 Liquid?Liquid Extraction (Small Molecules) (pages 557โ592): Karl Schugerl
Chapter 22 Protein Purification by Liquid?Liquid Extraction (pages 593โ616): Brian D. Kelley and T. Alan Hatton
Chapter 23 Protein Separation and Purification (pages 617โ642): Jan?Christer Janson and Lars Ryden
Chapter 24 Affinity Separations (pages 643โ677): Srikanth Sundaram and Martin L. Yarmush
Chapter 25 Electrokinetic Separations (pages 679โ693): Alan J. Grodzinsky and Martin L. Yarmush
Chapter 26 Final Recovery Steps: Lyophilization, Spray?Drying (pages 695โ714): Christian F. Golker
Chapter 27 Analytical Protein Chemistry (pages 717โ737): Srikanth Sundaram, David M. Yarmush and Martin L. Yarmush
Chapter 28 Biotechnology Facility Design and Process Validation (pages 739โ767): Michael G. Beatrice
Chapter 29 Treatment of Biological Waste (pages 769โ787): Daniel F. Liberman