Biosynthetic studies with carbon-13: Incorporation pattern of 1,2-13C-acetate into the fungal sesquiterpene ovalicin

✍ Scribed by Masato Tanabe; Kazuo T. Suzuki

• Publisher: Elsevier Science
• Year: 1974
• Tongue: French
• Weight: 155 KB
• Volume: 15
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(01)92178-1

✦ Synopsis

We report the use of doubly labelled acetate and FT-'aC-NMR in the determination of the biosynthetic origin of ovalicin (1) (1) (graphinone),a sesquiterpene found in cultures of Pseudeurotium ovalis.

The fungus was cultured in media fortified with 90% enriched 1,2-i3C-acetate that was diluted two-fold with unlabelled acetate. The FT-13C-NMR spectrum (Figure 1) of the labelled metabolite showed twelve characteristic (a) carbon satellite signals. These satellite signals which are caused by 13C-13C coupling occur on both sides of the center natural abundance peaks and only appear when doubly labelled acetic acid is incorporated. Since twelve such carbon