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Biosynthesis of the Cytochalasans. Part III. 13C-NMR. of cytochalasin B (phomin) and cytochalasin D. Incorporation of [1-13C]- and [2-13C]-sodium acetate

✍ Scribed by Werner Graf; Jean-Louis Robert; John C. Vederas; Christoph Tamm; Philippa H. Solomon; Iwao Miura; Koji Nakanishi

Publisher
John Wiley and Sons
Year
1974
Tongue
German
Weight
683 KB
Volume
57
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

Sequential single frequency decoupling and partially relaxed Fourier transform (PRFT) are used to assign the natural abundance ^13^C‐NMR. spectra of cytochalasin B (phomin) (1) and cytochalasin D (2). Cultures of Phoma spec. S 298 were fed [2‐^13^C]‐sodium acetate, and the distribution of this precursor in cytochalasin B (phomin) (1) was determined by ^13^C‐NMR. spectroscopy. Likewise, the labelling patterns in cytochalasin D (zygosporin A) (2) from Zygos‐posium masonii could be identified after incorporation of [2‐^13^C]‐sodium acetate and [l‐^13^C]‐sodium acetate. The results confirm previous proposals for the biogenesis of the cytochalasans from phenylalanine, methionine, and a C~18~, or C~16~, polyketide part.

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