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Biosynthesis of SOD by carrot hairy roots

✍ Scribed by Ji Hyeon Kim; Yong Woo Lee; Young Je Yoo

Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
327 KB
Volume
16
Category
Article
ISSN
0141-5492

No coin nor oath required. For personal study only.

✦ Synopsis

Superoxide dismutase(SOD) was produced by carrot hairy roots.

Mter increasing in the early exponential growth phase, SOD biosynthesis decreased as the growth rate of hairy roots declined. High sucrose concentration stimulated the SOD biosynthesis, while inhibiting the growth of hairy roots. Potential inducers were tested for the enhanced production of SOD. The addition of 0.3 mM CuSO4 to the broth showed about 6 fold increase in SOD biosynthesis compared to the results obtained with basic MS medium. Since CuSO4 in the broth inhibited the growth of hairy roots, the addition of CuSO4 to the broth at the late exponential growth phase led to a 12 times higher in~acellular level of SOD compared to that in basic MS medium.

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