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Biosynthesis of pullulan using immobilized Aureobasidium pullulans cells

โœ Scribed by Ashok Mulchandani; John H. T. Luong; Anh LeDuy

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
943 KB
Volume
33
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

Immobilization of Aureobasidium pullulans by adsorption on solid supports and entrapment in open pore polyurethane foam were attempted. By adsorption, the highest cell loading of 0.012-0.018 g dry wt/cm(2) support was obtained in pH 2.0 medium. Under this acidic condition, the net surface charges (zeta potentials) of both the cells and supports were close to zero and no pullulan was synthesized. Cationic coatings of Cytodex and polyethylenimine were not efficient in enhancing the binding strength between the cells and the supports. Surface immobilized cells and polyurethane foam entrapped cells exhibited a similar fermentation characteristics resulting in ca. 18 g/L pullulan and ca. 5 g/L leaked cells. However, cells entrapped in the polyurethane foam were more shear resistant. The immobilized cells thus could be repeatedly used for pullulan biosynthesis.

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