Immobilization of Aureobasidium pullulans by adsorption on solid supports and entrapment in open pore polyurethane foam were attempted. By adsorption, the highest cell loading of 0.012-0.018 g dry wt/cm(2) support was obtained in pH 2.0 medium. Under this acidic condition, the net surface charges (z
A pullulan-degrading enzyme activity of Aureobasidium pullulans
β Scribed by Dr. Thomas P. West; Beth Strohfus
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 271 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0233-111X
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β¦ Synopsis
A pullulan-degrading enzyme activity was detected in the culture medium after growth of the fungus Aureobasidium pullulans on corn syrup as a carbon source. The product of pullulan catabolism by this activity was determined to be glucose. The highest specific activity of this enzyme was found after 7 days of fungal growth. Although a-amylase activity was not detectable, the presence of a glucoamylase activity was indicated. It appeared that the enzyme responsible for pullulan degradation was likely glucoamylase B.
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