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Biosynthesis of penicillin V acylase byFusariumsp.: effect of culture conditions

✍ Scribed by V. K. Sudhakaran; J. G. Shewale

Publisher: Springer
Year: 1993
Tongue: English
Weight: 583 KB
Volume: 9
Category: Article
ISSN: 1573-0972
DOI: 10.1007/bf00327845

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✦ Synopsis

Penicillin V acylase was produced, both intracellularly and extracellularly, by Fusarium sp. SKF 235 grown in submerged fermentation. When neopeptone was added to the medium, >95% of the penicillin V acylase was extracellular. In the absence of a complex organic nitrogen source, the fungus produced low levels of totally intracellular penicillin V acylase. MgSO4 was essential for synthesis of the enzyme, which was induced by phenoxyacetic acid and penicillin V. The maximum yield of penicillin V acylase was 430 IU/g dry cell wt. The optimum pH value and temperature for the penicillin V acylase were 6.5 and 55°C, respectively.

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