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Biosynthesis of isoprenoids in Escherichia coli: Retention of the methyl H-atoms of 1-deoxy-D-xylulose

✍ Scribed by José-Luis Giner; Bernhard Jaun

Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
143 KB
Volume
39
Category
Article
ISSN
0040-4039

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✦ Synopsis

Investigation of isoprenoid biosynthesis in E. coli using [1,1,1-2H3]-l-deoxy-D-xylulose shows the methyl group of 1-deoxyxylulose to be incorporated into the methyl groups of ubiquinone originating from the methyl group of IPP and the (Z)-methyl group of DMAPP, and that this incorporation involves no loss of methyl hydrogen atoms.

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Optically pure 1-deoxy-D-xylulose, a key metabolite for feeding experiments in the methylerythritol phosphate pathway for isoprenoid biosynthesis, is conveniently synthesised from 1,2-O-isopropylidene-a-D-xylofuranose or from D-arabinose. This renders labelling with hydrogen isotopes possible.

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