Biosynthesis of gallotannins. Enzymatic conversion of 1,6-digalloylglucose to 1,2,6-trigalloylglucose

✍ Scribed by Klaus Denzel; Gerhard Schilling; Georg G. Gross

• Book ID: 104753085
• Publisher: Springer-Verlag
• Year: 1988
• Tongue: English
• Weight: 310 KB
• Volume: 176
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00392489

✦ Synopsis

Cell-free extracts from Rhus typhina L. (staghorn sumach) leaves were found to catalyze the transfer of the galloyl moiety of fl-glucogallin (1-O-galloyl-/%D-glucose) to 1,6-di-O-galloyl-fl-Dglucose, resulting in the specific formation of 1,2,6tri-O-galloyl-fl-D-glucose, an intermediate of gallotannin biosynthesis. The reaction product was unequivocally identified by co-chromatography with authentic references using reversed-phase high-performance liquid chromatography and by 1H-nuclear-magnetic-resonance spectroscopy.