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Biosynthesis of brassinolide from castasterone in cultured cells ofCatharanthus roseus

โœ Scribed by Hiroyuki Suzuki; Shozo Fujioka; Suguru Takatsuto; Takao Yokota; Noboru Murofushi; Akira Sakurai

Publisher
Springer
Year
1993
Tongue
English
Weight
475 KB
Volume
12
Category
Article
ISSN
0721-7595

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โœฆ Synopsis

Feeding experiments with tritium-and deuterium-labeled castasterone (CS) were conducted with three cell lines of Catharanthus roseus, including crown gall cells and nontransformed cells. In all three cell lines, the conversion of CS to brassinolide (BL) was observed and unequivocally confirmed by gas chromatography/mass spectrometry (GC/MS). This is the first conclusive evidence that CS is the biosynthetic precursor of BL.

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โœ Angela Stafford; Laura Smith; Michael W. Fowler ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 528 KB

Ceil suspension cultures of the Madagascan Periwinkle Catharanthus roseus (L) G. Don were maintained on Gamborg's B5 medium and their growth monitored by measuring cellular fresh and dry weight, ceil number and mitotic activity. Samples of cells of different ages and physiological states were subcul