๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Regulation of product synthesis in cell cultures ofCatharanthus roseus(L) G. Don

โœ Scribed by Angela Stafford; Laura Smith; Michael W. Fowler

Publisher
Springer
Year
1985
Tongue
English
Weight
528 KB
Volume
4
Category
Article
ISSN
0167-6857

No coin nor oath required. For personal study only.

โœฆ Synopsis

Ceil suspension cultures of the Madagascan Periwinkle Catharanthus roseus (L) G. Don were maintained on Gamborg's B5 medium and their growth monitored by measuring cellular fresh and dry weight, ceil number and mitotic activity. Samples of cells of different ages and physiological states were subcultured onto an alkaloid production medium and their rates of growth and alkaloid accumulation measured over a period of 30-45 days. In two experiments the rate of biomass accumulation was directly related to the rate of cellular serpentine accumulation. Possible mechanisms underlying this phenomenon are discussed in relation to the properties of cells comprising the inocula.

๐Ÿ“œ SIMILAR VOLUMES

Regulation of product synthesis in cell
Regulation of product synthesis in cell cultures ofCatharanthus roseus. V. Long-term maintenance of cells on a production medium
โœ P. Morris; K. Rudge; R. Cresswell; M. W. Fowler ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Springer ๐ŸŒ English โš– 565 KB

Three unselected cell lines of C. roseus maintained on a growth-associated alkaloid production medium were studied over a period of 2 to 5.5 years for the stability of alkaloid production (serpentine and ajmalicine). Large fluctuations in the total alkaloid content of 20-day-old cells were found for

Uptake and accumulation of ajmalicine in
Uptake and accumulation of ajmalicine into isolated vacuoles of cultured cells ofCatharanthus roseus(L.) G. Don. and its conversion into serpentine
โœ T. J. M. Blom; M. Sierra; T. B. Vliet; M. E. I. Franke-van Dijk; P. Koning; F. I ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 930 KB

Isolated vacuoles from ajmalicine-producing cell suspensions of Catharanthus roseus accumulated the alkaloid ajmalicine. Dissipation of the transtonoplast pH gradient with nigericin abolished ajmalicine accumulation, whereas dissipation of the transtonoplast potential with valinomycin had no effect.