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Biosynthesis of 35S-l-methionine of very high specific activity

โœ Scribed by Mark S. Bretscher; Alan E. Smith

Book ID
107711335
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
175 KB
Volume
47
Category
Article
ISSN
0003-2697

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## Abstract High specific activity Lโ€^35^ Sโ€cysteine and Lโ€^35^ Sโ€methionine were synthesised by using a wild type diploid strain of baker's __yeastโ€”Saccharomyces cerevisiae__. Yeast cells were grown in a sulphur depleted synthetic medium in which Na~2~ ^35^ SO~4~ (50 mCi/ml) was supplemented as th

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## Abstract The modified method for biosynthesis of Lโ€methionineโ€^35^S and Lโ€cystineโ€^35^S from baker's yeast (__Saccharomyces cerevisiae__) is described. The yeast was cultivated in a sulphurโ€deplated medium containing carrierโ€free Na~2~^35^SO~4~, under air bubbling during 24 hours. Yeast proteins