Bioseparation

✍ Scribed by Tsao G.T. (ed.)

Tongue: English
Leaves: 200
Edition: 1st edition
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This chapter is not intended to be a conventional review of gradient elution chromatography. Instead, the goal of this chapter is to provide practical strategies for large-scale separations using this method. Comprehensive reviews have provided its fundamentals and applications [1-4] for analytical purposes. In response to the increasing need for high purity bioproducts, advances in analytical liquid chromatography are being exploited for bioseparations [5]. Many of these bioproducts are proteins or other macro-molecules. However, most current theories and application strategies in gradient elution chromatography were developed for analytical purposes of small compounds, and they might not be appropriate for arge-scale separations of macro-molecules, which will be generally described in this section and in detail in the remaining sections of this chapter.

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