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Biosensors for enantioselective analysis

✍ Scribed by Thomas Kullick; Roland Ulber; Hartmut H. Meyer; Thomas Scheper; Karl Schügerl


Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
432 KB
Volume
293
Category
Article
ISSN
0003-2670

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✦ Synopsis


The development of enantioselective enzyme field effect transistors (EnFETs) for the detection of &hydroxy acid esters and aromatic amino esters and their application in flow-injection analysis is described. The EnFETs were produced by immobilising the enzyme on the pH-sensitive gate surface of a pH-FET. The characteristics of the sensor signals and their dependence on certain process parameters, such as pH, buffer capacity and flow conditions in the medium being measured, were examined. Due to the short response time, the EnFETs are ideal for application in flow-injection analysis. The use of enantioselective EnFETs in on-line process monitoring is described.


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