A postcolumn hqmd chromatographlc derwatlzatlon usmg a co-mnnoblhzed enzyme reactor was employed m the determmatlon of dextrms m wort and beer Amyloglucosldase, mutarotase and glucose dehydrogenase were co-munobtied on controlled-pore glass and packed m low dead volume columns The behavlour of the c
Bioselective detection in liquid chromatography by the use of immobilized enzymes
✍ Scribed by G. Marko-Varga; E. Domínguez; B. Hahn-Hägerdal; L. Gorton
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 425 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0731-7085
No coin nor oath required. For personal study only.
✦ Synopsis
The combination of liquid chromatography and immobilized enzyme reactors (LC/IMER) is a rapidly developing field of research. The enzymes are used to catalyse chemical reactions and thereby facilitate selective detection. In this instance the chemical derivatizations are performed in the post-column mode. The selectivity is demonstrated with respect to interfering compounds present in complex samples.
📜 SIMILAR VOLUMES
Assaying the low concentrations of steroid hormones in extracts of body fluids requires detectors that are both highly sensitive to the steroid and relatively insensitive to interfering compounds usually present in much higher concentrations. To explore the use of moderately specific enzymes in post