𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Bioreactor studies of the effect of medium pH on carrot (Daucus carotaL.) somatic embryogenesis

✍ Scribed by Véronique Jay; Simone Genestier; Jean-Claude Courduroux

Publisher
Springer
Year
1994
Tongue
English
Weight
347 KB
Volume
36
Category
Article
ISSN
0167-6857

No coin nor oath required. For personal study only.

✦ Synopsis

Daucus carom cell differentiation was examined under different medium pH conditions in a controlled bioreactor. Somatic embryogenesis was affected by pH changes. Embryo production was greatest when the pH of the hormone-free medium was maintained at 4.3. However, the same level was not favourable to development since most embryos did not progress to the torpedo and plantlet stages. In contrast, although there was about a threefold decrease in embryo yield in cultures on the same free 2,4-dichlorophenoxyacetic acid medium maintained at pH 5.8, cells differentiated into fully developed plantlets. Changes in embryo development appeared to be associated with alterations in ammonium loss from the medium and sugar uptake.

📜 SIMILAR VOLUMES

Bioreactor studies on the effect of diss
Bioreactor studies on the effect of dissolved oxygen concentrations on growth and differentiation of carrot (Daucus carotaL.) cell cultures
✍ Véronique Jay; Simone Genestier; Jean-Claude Courduroux 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 424 KB

A bioreactor control system was used to investigate the effects of two dissolved oxygen concentrations (10% and 100%) on the growth and differentiation of Daucus carota L. cell cultures. The strategy used allowed the dissolved oxygen concentration to be controlled without the need for changing eithe

Somatic proembryo production from excise
Somatic proembryo production from excised, wounded zygotic carrot embryos on hormone-free medium: evaluation of the effects of pH, ethylene and activated charcoal
✍ David L. Smith; Abraham D. Krikorian 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 981 KB

Wounded zygotic embryos of cultivated carrot produce somatic proembryos on hormone-free nutrient medium containing 1 mM NH 4 (+) as the sole nitrogen source. Continued maintenance of proembryos on this medium leads to a "pure" culture of preglobular stage proembryos (PGSPs). Ethylene had no effect o