Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

• Publisher: Wiley-Blackwell
• Year: 2007
• Tongue: English
• Leaves: 368
• Category: Library

✦ Synopsis

Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights.
Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.Content:
Chapter 1 High?Resolution Structures of Membrane Proteins: From X?Ray Crystallography to an Integrated Approach of Membranes (pages 1–27): Prof. Eva Pebay?Peyroula
Chapter 2 Membrane Protein Structure Determination by Electron Cryo?Microscopy (pages 29–54): Christopher G. Tate and John L. Rubinstein
Chapter 3 Introduction to Solid?State NMR and its Application to Membrane Protein–Ligand Binding Studies (pages 55–87): Krisztina Varga and Anthony Watts
Chapter 4 Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent (pages 89–120): Christine Ebel, Jesper V. Moller and Marc le Maire
Chapter 5 Probing Membrane Protein Interactions with Real?Time Biosensor Technology (pages 121–140): Iva Navratilova, David G. Myszka and Rebecca L. Rich
Chapter 6 Atomic Force Microscopy: High?Resolution Imaging of Structure and Assembly of Membrane Proteins (pages 141–158): Simon Scheuring, Nikolay Buzhynskyy, Rui Pedro Goncalves and Szymon Jaroslawski
Chapter 7 Molecular Dynamics Studies of Membrane Proteins: Outer Membrane Proteins and Transporters (pages 159–185): Syma Khalid, John Holyoake and Mark S. P. Sansom
Chapter 8 Understanding Structure and Function of Membrane Proteins Using Free Energy Calculations (pages 187–211): Christophe Chipot and Klaus Schulten
Chapter 9 Neutrons to Study the Structure and Dynamics of Membrane Proteins (pages 213–240): Kathleen Wood and Giuseppe Zaccai
Chapter 10 Circular Dichroism: Folding and Conformational Changes of Membrane Proteins (pages 241–258): Nadege Jamin and Jean?Jacques Lacapere
Chapter 11 Membrane Protein Structure and Conformational Change Probed using Fourier Transform Infrared Spectroscopy (pages 259–288): John E. Baenziger and Corrie J. B. daCosta
Chapter 12 Resonance Raman Spectroscopy of a Light?Harvesting Protein (pages 289–307): Andrew Aaron Pascal and Bruno Robert
Chapter 13 Energy Transfer Technologies to Monitor the Dynamics and Signaling Properties of G?Protein?Coupled Receptors in Living Cells (pages 309–334): Jean?Philippe Pin, Mohammed?Akli Ayoub, Damien Maurel, Julie Perroy and Eric Trinquet