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Biophotonic cytotoxicity assay for high-throughput screening of cytolytic killing

✍ Scribed by Christine E. Brown; Christine L. Wright; Araceli Naranjo; Reena P. Vishwanath; Wen-Chung Chang; Simon Olivares; Jamie R. Wagner; Laura Bruins; Andrew Raubitschek; Laurence J.N. Cooper; Michael C. Jensen


Book ID
113716921
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
478 KB
Volume
297
Category
Article
ISSN
0022-1759

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We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur