On-line monitoring of intracellular ATP
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Daniel R Lasko; Daniel I. C. Wang
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Article
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2000
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John Wiley and Sons
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English
β 972 KB
A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a cons