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Bioluminescent monitoring of intracellular ATP during fermentation

✍ Scribed by Hisakage Funabashi; Toshiya Imajo; Junichiro Kojima; Eiry Kobatake; Masuo Aizawa


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
96 KB
Volume
14
Category
Article
ISSN
1522-7235

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On-line monitoring of intracellular ATP
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A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a cons