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Automatic monitoring of protease activity during fermentation

✍ Scribed by Matti Leisola; Heikki Ojamo; Veli Kauppinen


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
135 KB
Volume
1
Category
Article
ISSN
0141-0229

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An assay for the detection of yeast (Saccharomyces cerevisiae) protease activity, using partially puri- fied yeast-derived recombinant hepatitis B surface antigen (rHBsAg) as substrate, was developed to monitor proteolysis of rHBsAg that may occur through fermentation and isolation. The method consi