Bioluminescence and Chemiluminescence: Progress and Perspectives

✍ Scribed by Tsuji A., Matsumoto M., Maeda M.

• Year: 2004
• Tongue: English
• Leaves: 599
• Category: Library

✦ Synopsis

Synopsis''Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence.The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays.''

✦ Table of Contents

9812561188......Page 1
CONTENTS......Page 16
Preface......Page 8
PART 1. BIOLUMINESCENCE......Page 30
MATERIALS AND METHODS......Page 32
RESULTS......Page 33
DISCUSSION......Page 34
REFERENCES......Page 35
INTRODUCTION......Page 36
Fluorinated dehydrocoelenterazine......Page 37
ACKNOWLEDGEMENTS......Page 38
REFERENCES......Page 39
RESULTS AND DISCUSSION......Page 40
REFERENCES......Page 43
INTRODUCTION......Page 44
The four conserved histidines are found in all seven species examined......Page 45
Structure of domain 3 of L. polyedrum giving the location of histidines......Page 46
REFERENCES......Page 47
LUMINESCENCE IN ECHINODERMS......Page 48
LUMINESCENCE IN OPHIUROIDS......Page 49
ACKNOWLEDGMENTS......Page 50
REFERENCES......Page 51
Habitat......Page 52
Cessation of luminescence......Page 53
Luminescence activity......Page 54
REFERENCES......Page 55
Aequorin and GFP: an historical account Shimomura O......Page 56
REFERENCES......Page 62
INTRODUCTION......Page 64
RESULTS AND DISCUSSION......Page 65
ACKNOWLEDGMENTS......Page 66
REFERENCES......Page 67
METHODS......Page 68
Electrophysiology......Page 69
DISCUSSION......Page 70
REFERENCES......Page 71
PART 2. BEETLE BIOLUMINESCENCE......Page 72
INTRODUCTION......Page 74
Concentration dependency of the N-domain activity......Page 75
Sensitive detection of LHz-AMP using the N-domain......Page 76
REFFERENCES......Page 77
INTRODUCTION......Page 78
Dependence of product yield on enzyme and substrate concentration......Page 79
REFERENCES......Page 81
MATERIALS AND METHODS......Page 82
RESULTS AND DISCUSSION......Page 83
REFERENCES......Page 85
EXPERIMENTAL METHODS......Page 86
RESULTS AND DISCUSSION......Page 87
REFERENCES......Page 89
METHODS......Page 90
RESULTS AND DISCUSSION......Page 91
REFERENCES......Page 93
METHODS......Page 94
RESULTS......Page 95
REFERENCES......Page 97
Absorption and fluorescence spectra of MMOL......Page 98
Stability of DMOL and MMOL in buffer solutions......Page 99
Interaction of MMOL and DMOL with the wild-type and mutant luciferases......Page 100
REFERENCES......Page 101
PART 3. MARINE BACTERIA BIOLUMINESCENCE......Page 102
MATERIALS AND METHODS......Page 104
RESULTS AND DISCUSSION......Page 105
REFERENCES......Page 107
THEORY......Page 108
REFERENCES......Page 111
INTRODUCTION......Page 112
RESULTS AND DISCUSSION......Page 113
REFERENCES......Page 115
METHODS......Page 116
RESULTS......Page 117
REFERENCES......Page 119
METHODS......Page 120
RESULTS......Page 121
REFERENCES......Page 123
LUMINOUS BACTERIA AS PRODUCERS OF ENZYMES......Page 124
DATABASE OF CULTURE COLLECTION IBSO......Page 126
REFERENCES......Page 127
INTRODUCTION......Page 128
RESULTS AND DISCUSSION......Page 129
REFERENCES......Page 131
Isolation of P. leiognathi from the light organ of L. rivulatus......Page 132
RESULTS AND DISCUSSION......Page 133
REFERENCES......Page 135
Preparation of supplements......Page 136
Effect of amino acids and chitosan on RLU of RL-1......Page 137
Effect of amino acids and chitosan on RLU of other strains......Page 138
REFERENCES......Page 139
PART 4. CYPRIDINA (VARGULA) BIOLUMINESCENCE......Page 140
INTRODUCTION......Page 142
RESULTS AND DISCUSSION......Page 143
REFERENCES......Page 145
RESULTS AND DISCUSSION......Page 146
REFERENCES......Page 149
MATERIALS AND METHODS......Page 150
RESULTS AND DISCUSSION......Page 151
REFERENCES......Page 153
MATERIALS AND METHODS......Page 154
RESULTS AND DISCUSSION......Page 155
CONCLUSION......Page 156
REFERENCES......Page 157
INTRODUCTION......Page 158
RESULTS AND DISCUSSION......Page 159
REFERENCES......Page 161
PART 5. CHEMILUMINESCENCE......Page 162
INTRODUCTION......Page 164
Reaction of DMD with tertiary amines......Page 165
Effect of DABCO on the singlet oxygen dimol emission......Page 166
REFERENCES......Page 167
METHODS......Page 168
RESULTS AND DISCUSSION......Page 169
REFERENCES......Page 171
Chemiluminescence of the uranium compounds4......Page 172
Chemiluminescence of lanthanide chelates during decomposition of dioxetanes ......Page 173
REFERENCES......Page 175
Apparatus and Procedure......Page 176
RESULTS AND DISCUSSIONS......Page 177
REFERENCES......Page 179
Apparatus and Procedure......Page 180
RESULTS AND DISCUSSION......Page 181
REFERENCES......Page 183
INTRODUCTION......Page 184
RESULTS......Page 185
REFERENCES......Page 187
RESULTS AND DISCUSSIONS......Page 188
REFERENCES......Page 191
RESULTS......Page 192
DISCUSSIONS......Page 194
REFERENCES......Page 195
COMPUTATIONAL METHODS......Page 196
Theoretical interpretation of the large difference in the quantum yield of luminescence between DD and DHA......Page 197
REFERENCES......Page 199
RESULTS AND DISCUSSION......Page 200
REFERENCES......Page 203
CL measurements of indole, I and II......Page 204
Chemiluminescence......Page 205
REFERENCES......Page 207
Apparatus......Page 208
RESULTS......Page 209
REFERENCES......Page 211
RESULTS AND DISCUSSION......Page 212
REFERENCES......Page 214
Materials......Page 216
Measurement of CL spectra (heated samples)......Page 217
Effect of the preservation temperature on chemiluminescence......Page 218
REFERENCES......Page 219
EXPERIMENTAL......Page 220
Metallic salts......Page 221
Application to Hz02 analysis......Page 222
REFERENCES......Page 223
PART 6. 1,ZDIOXETANES......Page 224
RESULTS AND DISCUSSION......Page 226
REFERENCES......Page 229
RESULTS AND DISCUSSION......Page 230
REFERENCES......Page 233
Charge and electron transfer......Page 234
Charge density in PHOD anion......Page 235
HOMO suggests nature of excited state......Page 236
REFERENCES......Page 237
Intramolecular CIEEL mechanism on chemiluminescence of phenoxide substituted 1, 2-dioxetanes Tanaka C, Tanaka J and Matsumoto M......Page 238
REFERENCES......Page 241
PART 7. INSTRUMENTATION & DEVICES......Page 242
Instrumentation......Page 244
RESULTS......Page 245
REFERENCES......Page 247
scanning near-field opWatomic force microscope (SNOWAFM)......Page 248
DNA solution and stretching method......Page 249
Detection of PNA pmbe on DNA molecule......Page 250
REFERENCES......Page 251
INSTRUMENTATION DEVELOPMENT......Page 252
Reagents......Page 253
DISCUSSION......Page 254
REFERENCES......Page 255
PART 8. APPLICATIONS OF LUMINESCENCE......Page 256
INTRODUCTION......Page 258
Measurement of Ara-C activity......Page 259
REFERENCES......Page 260
Reagents and samples......Page 262
Detection......Page 263
RESULTS......Page 264
REFERENCE......Page 265
RESULTS......Page 266
ACKNOWLEDGMENTS......Page 268
REFERENCES......Page 269
Chemicals......Page 270
RESULTS AND DISCUSSION......Page 271
REFERENCES......Page 273
Materials and reagents......Page 274
Optimization of UV irradiation......Page 275
Optimization of CL condition......Page 276
REFERENCES......Page 277
Tagged expression in mammalian cells and cell culture conditions......Page 278
In vitro transcription and translation of Photinam......Page 279
Recombinant Photinam and measurement of light production......Page 280
REFERENCES......Page 281
DESIGN OF FLUORESCENCE PROBES BASED ON PHOTOINDUCED ELECTRON TRANSFER......Page 282
FLUORESCENCE PROBES BASED ON BORON DIPYRROMETHENE......Page 284
REFERENCES......Page 285
HPLC system and chromatographic conditions......Page 286
Derivatization conditions with DIB-CI......Page 287
Method validation......Page 288
REFERENCES......Page 289
METHODS......Page 290
RESULTS......Page 291
REFERENCES......Page 293
Principles......Page 294
Experiments......Page 295
RESULTS......Page 296
REFERENCES......Page 297
Materials......Page 298
RESULTS......Page 299
REFERENCES......Page 301
METHODS......Page 302
RESULTS AND DISCUSSION......Page 304
REFERENCES......Page 305
PART 9. ANTIOXIDANTS, REACTIVE OXYGEN SPECIES & PHAGOCYTOSIS......Page 306
MATERIAL AND METHODS......Page 308
RESULTS......Page 309
ACKNOWLEDGMENTS......Page 311
METHODS......Page 312
Analyte in gaseous phase......Page 313
REFERENCES......Page 315
Neutrophil chemiluminescence......Page 316
RESULTS......Page 317
DISCUSSION......Page 318
REFERENCES......Page 319
MATERIALS AND METHODS......Page 320
RESULTS AND DISCUSSION......Page 321
REFERENCES......Page 323
Isolation of peroxisomes......Page 324
Respiration and ROS generation in mitochondria......Page 325
ROS generation in peroxisomes......Page 326
REFERENCES......Page 327
MATERIALS AND METHODS......Page 328
RESULTS AND DISCUSSION......Page 329
REFERENCES......Page 331
INTRODUCTION......Page 332
RESULTS......Page 333
REFERENCES......Page 334
MATERIALS AND METHODS......Page 336
RESULTS AND DISCUSSION......Page 337
REFERENCES......Page 339
Chemiluminescence measurement......Page 340
RESULTS AND DISCUSSION......Page 341
REFERENCES......Page 343
MATERIALS AND METHODS......Page 344
RESULTS AND DISCUSSION......Page 345
REFERENCES......Page 347
Preparation of sample solution for detection of HzOz in plants extract......Page 348
Antibacterial effect of natural plant extract......Page 349
Generation of Hz02 from natural plant extract......Page 350
REFERENCES......Page 351
All-trans retinoic acid and monomers treatment......Page 352
Cell viability and inhibition of cell growth......Page 353
DISCUSSION......Page 354
REFERENCES......Page 355
EXPERIMENTAL......Page 356
RESULTS AND DISCUSSION......Page 357
REFERENCES......Page 359
MATERIALS AND METHODS......Page 360
RESULTS AND DISCUSSION......Page 361
REFERENCES......Page 363
MATERIALS AND METHODS......Page 364
RESULTS AND DISCUSSION......Page 365
REFERENCES......Page 367
Instrument and measurement principle......Page 368
RESULTS AND DISCUSSION......Page 370
REFERENCES......Page 371
GENERATION METHODS OF SINGLET OXYGEN......Page 372
APPLICATIONS OF SINGLET OXYGEN TO STERILIZATION/ PURIFICATION......Page 373
ACKNOWLEDGEMENTS......Page 374
REFERENCES......Page 375
General procedure for synthesis and analysis of probes......Page 376
RESULTS AND DISCUSSION......Page 377
REFERENCES......Page 379
Subjects, blood collection and PMN isolation......Page 380
RESULTS......Page 381
DISCUSSION AND CONCLUSIONS......Page 382
REFERENCES......Page 383
METHODS......Page 384
RESULTS......Page 385
REFERENCES......Page 387
INTRODUCTION......Page 388
RESULTS AND DISCUSSION......Page 390
REFERENCES......Page 391
PART 10. APPLICATIONS IN MICROBIOLOGY, ECOLOGY, AND ENVIRONMENTAL & FOOD TESTING......Page 392
Phagocytosis of S. Typhimurium DT104 lux' by THP-1 cell......Page 394
Assay to assess phagocytosis of S. Typhimurium DT104 Zux' by THP-1 cells......Page 395
DISCUSSION......Page 396
REFERENCES......Page 397
Construction of self-bioluminescent reporter strains......Page 398
RESULTS AND DISCUSSION......Page 399
REFERENCES......Page 401
Bioluminescent detection......Page 402
Concentration dependent effects of H202 on bioluminescence......Page 403
DMSO Enhances the delivery of H202 and the promotion of bioluminescence......Page 404
REFERENCES......Page 405
INTRODUCTION......Page 406
CONCLUSIONS......Page 407
ACKNOWLEDGMENTS......Page 408
References......Page 409
Bacterial strains and chemical reagents......Page 410
RESULTS AND DISCUSSION......Page 411
REFERENCES......Page 413
MATERIALS AND METHODS......Page 414
RESULTS......Page 415
REFERENCES......Page 417
METHODS......Page 418
RESULTS......Page 419
REFERENCES......Page 421
Inhibiting growth of Escherichia coli 3-5......Page 422
Inhibiting growth of Escherichiu coli......Page 423
Decontaminating water-bloom......Page 424
REFERENCES......Page 425
EXPERIMENTAL......Page 426
RESULTS AND DISCUSSION......Page 427
REFERENCES......Page 429
Sample......Page 430
RESULTS AND DISCUSSION......Page 431
REFERENCES......Page 433
EXPERIMENTAL......Page 434
RESULTS AND DISCUSSION......Page 435
REFERENCES......Page 437
METHODS......Page 438
RESULTS......Page 439
REFERENCES......Page 441
METHODS......Page 442
Bioluminescent ecological monitoring of salt lake......Page 443
System of biosensors for ecological monitoring......Page 444
REFERENCES......Page 445
Instrumentation......Page 446
Methods......Page 447
RESULTS......Page 448
REFERENCES......Page 449
GLASS MICROCHIP FOR TRANSITION METAL ION ANALYSIS......Page 450
A MICROCHIP BASED ON THE OXALATE-H202 CL SYSTEM......Page 451
SEPARATION AND CL DETECTION OF DOPAMINE AND CATECHOL......Page 452
REFERENCES......Page 453
IMPROVED ATP REAGENTS......Page 454
EXTRACTION OF INTRACELLULAR ATP......Page 455
ALTERNATIVES TO TRADITIONAL ATP ASSAYS......Page 456
REFERENCES......Page 457
INTRODUCTION......Page 458
RESULTS & DISCUSSION......Page 459
REFERENCES......Page 461
BIOLUMINESCENT BIOREPORTER INTEGRATED CIRCUITS (BBICS)......Page 462
BBIC SENSING OF THE CHEMICAL ENVIRONMENT......Page 463
BIOREPORTER SENSING OF BIOLOGICAL AGENTS......Page 464
REFERENCES......Page 465
INTRODUCTION......Page 466
Detection of bioluminescence in BOD standard solutions......Page 467
REFERENCES......Page 468
INTRODUCTION......Page 470
Improvement of the HPLC system......Page 471
Application to airborne particulates......Page 472
REFERENCES......Page 473
METHODS......Page 474
RESULTS......Page 476
REFERENCES......Page 477
A. NASA APPLICATIONS OF THE ATP-BASED ASSAY......Page 478
B. ADVANTAGES AND DISADVANTAGES......Page 480
REFERENCES......Page 481
Measurement of emission spectra......Page 482
Imaging of ROS, hydrogen donor and mediator......Page 483
Application to food analysis......Page 484
REFERENCES......Page 485
PART 11. LUMINESCENT IMMUNOASSAYS......Page 486
INTRODUCTION......Page 488
Simultaneous BL-EIA for BDNF and NT-4/5......Page 489
REFERENCES......Page 490
Calcium-regulated photoprotein obelin as a label in immunoassay: an outlook for applications Frank LA, Borisova Wand Vysotski ES......Page 492
REFERENCES......Page 495
Assay procedure......Page 496
Cross-reactivity......Page 497
Method comparison......Page 498
REFERENCES......Page 499
Tandem BL-EIA for Angiotensin I and Endothelin-1......Page 500
RESULTS AND DISCUSSION......Page 501
REFERENCE......Page 503
A HIGH-THROUGHPUT METHOD FOR DEVELOPMENT OF FRET- BASED INDICATORS FOR PROTEOLYSIS......Page 504
EXPANDED DYNAMIC RANGE OF FRET-BASED Ca^ INDICATORS BY CIRCULARLY PERMUTED YELLOW FLUORESCENT PROTEINS......Page 505
REFERENCES......Page 507
INTRODUCTION......Page 508
Assay Format......Page 509
RESULTS AND DISCUSSION......Page 510
REFERENCES......Page 511
METHODS & RESULTS......Page 512
REFERENCES......Page 515
METHODS & RESULTS......Page 516
REFERENCES......Page 519
MATERIALS AND METHODS......Page 520
RESULTS AND DISCUSSION......Page 521
REFERENCES......Page 523
Avidin-biotin chemiluminescence immunoassay procedure......Page 524
The optimization of solid phase conditions......Page 525
Comparison with Radioimmunoassay (RIA)......Page 526
REFERENCES......Page 527
PART 12. LUMINESCENT ASSAYS FOR ENZYMES, SUBSTRATES, INHIBITORS & CO-FACTORS......Page 528
MATERIALS AND METHODS......Page 530
RESULTS AND DISCUSSION......Page 531
REFERENCES......Page 533
Reagents and solutions......Page 534
Optimization of assay condition......Page 535
CONCLUSION......Page 536
REFERENCES......Page 537
Simultaneous bioluminescent assay for aequorin and biotinylated luciferase......Page 538
Simultaneous bioluminescent assay for aequorin and biotinylated luciferase......Page 539
REFERENCES......Page 541
INTRODUCTION......Page 542
Principles for accumulated light detection by HRP catalyzed imidazole CL......Page 543
REFERENCES......Page 544
PART 13. LUMINESCENT DNA PROBE, GENE EXPRESSION & REPORTER GENE ASSAYS......Page 546
PCR......Page 548
RESULTS AND DISCUSSION......Page 549
Bioluminescent detection of Allele specific PCR products......Page 550
ACKNOWLEDGEMENTS......Page 551
INTRODUCTION......Page 552
RESULTS......Page 553
REFERENCES......Page 555
METHODS......Page 556
RESULTS......Page 557
DISCUSSION......Page 558
REFERENCES......Page 559
INTRODUCTION......Page 560
RESULTS......Page 561
CONCLUSION......Page 562
REFERENCES......Page 563
METHODS......Page 564
Efficiency of split Renillu luciferase complementation......Page 565
Time course of the interaction between Y941 and SH2n......Page 566
REFERENCES......Page 567
Reapents:......Page 568
RESULTS......Page 569
REFERENCES......Page 571
MATERIALS AND METHODS......Page 572
DISCUSSION......Page 573
ACKNOWLEDGEMENTS......Page 574
REFERENCES......Page 575
METHODS......Page 576
RESULTS......Page 577
DISCUSSIONS......Page 578
REFERENCES......Page 579
Index......Page 580