Bioluminescence and Chemiluminescence Part C

• Publisher: Academic Press
• Year: 2000
• Tongue: English
• Leaves: 764
• Series: Methods in Enzymology 305
• Edition: 1
• Category: Library

✦ Synopsis

Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels of light continue to push the limits of detection, allowing ready monitoring in real time of intricate subcellular processes within living cells. This book affords a glimpse of the state of the art of a rapidly advancing field, and presents to users of these methods a detailed reference of current activities in the field.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features
@bul Outlines the use of luminescent reporter technologies to monitor gene expression and protein trafficking
Describes the luminescence-based clinical assay technologies
Details the basic biochemistry, biophysics, and chemistry of light-emitting reactions that are critical for applications
Includes explanations of the instrumentation used for detection and quantification of low level light
* Shows the new applications of luminescence-based technologies that result largely from broad advances in recombinant DNA technologies and nonscale methods

✦ Table of Contents

Content:
Contributors to volume 305
Pages xi-xiv

Preface
Pages xv-xvi
Miriam M. Ziegler, Thomas O. Baldwin

Methods in enzymology
Pages xvii-xxxv

Chemical generation of excited states: The basis of chemiluminescence and bioluminescence Original Research Article
Pages 3-47
Frank McCapra

Some brief notes on nomenclature and units and standards used in bioluminescence and chemiluminescence Original Research Article
Pages 47-50
Philip E. Stanley

Physics of low light level detectors Original Research Article
Pages 53-61
Kenneth J. Voss

Luminometer design and low light detection Original Research Article
Pages 62-87
Fritz Berthold, Klaus Herick, Ruth M. Siewe

Absolute calibration of luminometers with low-level light standards Original Research Article
Pages 87-96
Dennis J. O'Kane, John Lee

Commercially available luminometers and low-level light imaging devices Original Research Article
Pages 96-103
Philip E. Stanley

Automated recordings of bioluminescence with special reference to the analysis of circadian rhythms Original Research Article
Pages 104-119
Till Roenneberg, Walter Taylor

Chemiluminescence imaging systems for the analysis of macrosamples: Microtiter format, blot membrane, and whole organs Original Research Article
Pages 120-132
Aldo Roda, Patrizia Pasini, Monica Musiani, Mario Baraldini

Overexpression of bacterial luciferase and purification from recombinant sources Original Research Article
Pages 135-152
Thomas O. Baldwin, Miriam M. Ziegler, Vicki A. Green, Michael D. Thomas

Purification of luciferase subunits from recombinant sources Original Research Article
Pages 152-157
James F. Sinclair

A rapid chromatographic method to separate the subunits of bacterial luciferase in urea-containing buffer Original Research Article
Pages 157-164
A.Clay Clark, Brian W. Noland, Thomas O. Baldwin

Purification and ligand exchange protocols for antenna proteins from bioluminescent bacteria Original Research Article
Pages 164-180
Valentin N. Petushkov, Bruce G. Gibson, Antonie J.W.G. Visser, John Lee

Purification of firefly luciferase from recombinant sources Original Research Article
Pages 180-188
Thomas O. Baldwin, Vicki A. Green

Chemical synthesis of firefly luciferin analogs and inhibitors Original Research Article
Pages 188-195
Bruce R. Branchini

Structural basis for understanding spectral variations in green fluorescent protein Original Research Article
Pages 196-211
S.James Remington

Large-scale purification of recombinant green fluorescent protein from Escherichia coli Original Research Article
Pages 212-223
Daniel G. GonzГЎlez, William W. Ward

Recombinant obelin: Cloning and expression of cDNA, purification, and characterization as a calcium indicator Original Research Article
Pages 223-249
Boris A. Illarionov, Ludmila A. Frank, Victoria A. Illarionova, Vladimir S. Bondar, Eugene S. Vysotski, John R. Blinks

Gonyaulax luciferase: Gene structure, protein expression, and purification from recombinant sources Original Research Article
Pages 249-258
Liming Li

Dinoflagellate luciferin-binding protein Original Research Article
Pages 258-276
David Morse, Maria Mittag

Assay of autoinducer activity with luminescent Escherichia coli sensor strains harboring a modified Vibrio fischeri lux regulon Original Research Article
Pages 279-287
Jerry H. Devine, Gerald S. Shadel

Detection, purification, and structural elucidation of the acylhomoserine lactone inducer of Vibrio fischeri luminescence and other related molecules Original Research Article
Pages 288-301
Amy L. Schaefer, Brian L. Hanzelka, Matthew R. Parsek, E.Peter Greenberg

Chemical synthesis of bacterial autoinducers and analogs Original Research Article
Pages 301-315
Anatol Eberhard, Jeffrey B. Schineller

Overexpression of foreign proteins using the Vibrio fischeri lux control system Original Research Article
Pages 315-329
Michael D. Thomas, Anita Van Tilburg

Application of bioluminescence and chemiluminescence in biomedical sciences Original Research Article
Pages 333-345
Larry J. Kricka

Use of firefly luciferase in atp-related assays of biomass, enzymes, and metabolites Original Research Article
Pages 346-370
Arne Lundin

Chemiluminescent methods for detecting and quantitating enzyme activity Original Research Article
Pages 370-390
Larry J. Kricka, John C. Voyta, Irena Bronstein

Chemiluminescence assay of serum alkaline phosphatase and phosphoprotein phosphatases Original Research Article
Pages 391-401
Brenda J. Hallaway, Dennis J. O'Kane

Chemiluminescence screening assays for erythrocytes and leukocytes in urine Original Research Article
Pages 402-410
Valerie J. Bush, Brenda J. Hallaway, Thomas A. Ebert, David M. Wilson, Dennis J. O'Kane

Immunoassay protocol for quantitation of protein kinase activities Original Research Article
Pages 410-416
Jennifer Mosier, Corinne E.M. Olesen, John C. Voyta, Irena Bronstein

Chemiluminescent immunodetection protocols with 1,2-dioxetane substrates Original Research Article
Pages 417-427
Corinne E.M. Olesen, Jennifer Mosier, John C. Voyta, Irena Bronstein

Chemiluminescent reporter gene assays with 1,2-dioxetane enzyme substrates Original Research Article
Pages 428-450
Corinne E.M. Olesen, Chris S. Martin, Jennifer Mosier, Betty Liu, John C. Voyta, Irena Bronstein

Clinical application of southern blot hybridization with chemiluminescence detection Original Research Article
Pages 450-466
Kathleen S. Tenner, Dennis J. O'Kane

Quantitative polymerase chain reaction and solid-phase capture nucleic acid detection Original Research Article
Pages 466-476
Chris S. Martin, John C. Voyta, Irena Bronstein

Targeted bioluminescent indicators in living cells Original Research Article
Pages 479-498,IN1
Graciela B. Sala-Newby, Michael N. Badminton, W.Howard Evans, Christopher H. George, Helen E. Jones, Jonathan M. Kendall, Angela R. Ribeiro, Anthony K. Campbell

Green fluorescent protein as a reporter of transcriptional activity in a prokaryotic system Original Research Article
Pages 499-513
Deborah A. Siegele, Lisa Campbell, James C. Hu

Bacterial lux genes as reporters in cyanobacteria Original Research Article
Pages 513-527
F. FernГЎndez-PiГ±as, F. LeganГ©s, C.Peter Wolk

Application of bioluminescence to the study of circadian rhythms in cyanobacteria Original Research Article
Pages 527-542
Carol R. Andersson, Nicholas F. Tsinoremas, Jeffrey Shelton, Nadya V. Lebedeva, Justin Yarrow, Hongtao Min, Susan S. Golden

Construction of lux bacteriophages and the determination of specific bacteria and their antibiotic sensitivities Original Research Article
Pages 543-557
S. Ulitzur, J. Kuhn

Luciferase gene as reporter: Comparison with the CAT gene and use in transfection and microinjection of mammalian cells Original Research Article
Pages 557-576
S. Gelmini, P. Pinzani, M. Pazzagli

In situ hybridization and immunohistochemistry with enzyme-triggered chemiluminescent probes Original Research Article
Pages 577-586,IN3-IN4,587-590
Aldo Roda, Monica Musiani, Patrizia Pasini, Mario Baraldini, Jean E. Crabtree

Blood phagocyte luminescence: gauging systemic immune activation Original Research Article
Pages 591-629
Robert C. Allen, David C. Dale, Fletcher B. Taylor Jr.

Demonstrations of chemiluminescence Original Research Article
Pages 633-659
Frank McCapra

Bioluminescence as a classroom tool for ccientist volunteers Original Research Article
Pages 660-672
Mara Hammer, Joseph D. Andrade

Green fluorescent protein in biotechnology education Original Research Article
Pages 672-680
William W. Ward, Gavin C. Swiatek, Daniel G. Gonzalez

Author index
Pages 681-713

Subject index
Pages 715-732