Biological properties of a kringleless tissue plasminogen activator (t-PA) mutant
โ Scribed by Ehrlich, H.J.; Bang, N.U.; Little, S.P.; Jaskunas, S.R.; Weigel, B.J.; Mattler, L.E.; Harms, C.S.
- Book ID
- 122651852
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 741 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0268-9499
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A protease-deficient strain of Aspergillus niger has been used as a host for the production of human tissue plasminogen activator (t-PA). In defined medium, up to 0.07 mg t-PA (g biomass)(-1) was produced in batch and fed-batch cultures and production was increased two- to threefold in two-phase bat
Full-length tissue-type plasminogen activator (t-PA) cDNA served to construct deletion mutants within the N-terminal "heavy" (H)-chain of the t-PA molecule. The H-chain cDNA consists of an array of structural domains homologous to domains present on other plasma proteins ("finger, " "epidermal growt