Biological Membranes: Structure, Biogenesis and Dynamics

✍ Scribed by William Dowhan (auth.), Jos A. F. Op den Kamp (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1994
• Tongue: English
• Leaves: 357
• Series: NATO ASI Series 82
• Edition: 1
• Category: Library

✦ Synopsis

The Advanced Study Institute on "Structure, Biogenesis and Dynamics of Biological Membranes, held in Cargese from June 14-26, 1993, has been dealing with four major topics in membrane biochemistry today: lipid dynamics and lipid-protein interactions, protein translocation and insertion, intracellular traffic aud protein structure and folding. The lecturers discussed these topics starting from several disciplines, including biochemistry, cell biology, genetics, and biophysics. This wayan interdisciplinary and very inte~sting view on biological membrane systems was obtained. At first an extensive overview of -mainly biophysical -techniques which can be used to study dynamic processes in membranes was presented. Sophisticated approaches such as ESR and NMR have been applied succesfully to unravel details of specific lipid-protein interactions. x­ ray analysis provides detailed structural information of several proteins and the possible implications for protein functions. Information obtained this way is complemented by studies on mechanisms and kinetics of protein folding. The latter information is indispensable when discussing protein translocation and insertion: proces:;es in which folding and unfolding play essential roles. Extensive insight was offered in the complicated machinery of phospholipid biosynthesis. In particular, the application of sophisticated genetic techniques has allowed a better understanding of the mechanisms regulating the synthetic machinery and detailed studies on a variety of mutants, lacking one or more of the essential enzymes, have resulted in the beginning of a bL!:

✦ Table of Contents

Front Matter....Pages I-X
Role of Phospholipids in Cell Function....Pages 1-21
Assembly of Sphingolipids into Membranes of the Yeast, Saccharomyces Cerevisiae....Pages 23-31
Localization of Phospholipids in Plasma Membranes of Mammalian Cells....Pages 33-41
Some Aspects of Intracellular Lipid Traffic....Pages 43-51
Intracellular Localization and Mechanisms of Regulation of Phosphatidylinositol Transfer Protein in Swiss Mouse 3T3 Cells....Pages 53-59
Phosphatidylinositol Transfer Protein Function in Yeast....Pages 61-70
Conversion of the amphiphilic 115 kDa Form of Glycosyl-Phosphatidylinositol-specific Phospholipase D to an active, hydrophilic 47 kDa Form....Pages 71-78
Magnetic Resonance Studies of Protein-Lipid Interactions....Pages 79-92
Lipid-Protein Interactions with the Hydrophobic SP-B and SP-C Lung Surfactant Proteins in Dipalmitoylphosphatidylcholine Bilayers....Pages 93-100
Protein-Membrane Interactions in the Complex Biological Milieu....Pages 101-106
Protein Sorting and Glycolipid-Enriched Detergent-Insoluble Complexes in Epithelial Cells....Pages 107-114
Structural and Functional Consequences of Acylation of a Transmembrane Peptide....Pages 115-130
Non-Crystallographic Methods to Study Membrane Proteins....Pages 131-138
Preferred Local Conformations of Peptides comprising the Entire Sequence of Bovine Pancreatic Trypsin Inhibitor (BPTI) and their Roles in Protein Folding....Pages 139-153
Co-translational Modification, Stability and Turnover of Eukaryotic Proteins....Pages 155-167
Structure and Mechanism of Porins....Pages 169-179
The Pore-Forming Toxin Aerolysin: From the Soluble to a Transmembrane Form....Pages 181-190
Signal Sequences: Roles and Interactions by Biophysical Methods....Pages 191-198
Assembly of Integral Membrane Proteins....Pages 199-205
How do Proteins Cross a Membrane?....Pages 207-214
Protease secretion by Erwinia chrysanthemi and Serratia marcescens ....Pages 215-222
ScFv Antibody Fragments Produced in Saccharomyces cerevisiae Accumulate in the Endoplasmic Reticulum and the Vacuole....Pages 223-236
Preprotein Binding by ATP-Binding Site Mutants of the Bacillus Subtilis SecA....Pages 237-244
Internal Disulfides in the Diphteria Toxin A-Fragment Block Its Translocation to the Cytosol....Pages 245-251
Molecular Characterization, Assembly and Membrane Association of the Groel-Type Chaperonins in Synechocystis PCC 6803....Pages 253-261
Identification and Characterization of Novel ATP-Binding Cassette Proteins in Saccharomyces Cerevisiae ....Pages 263-268
Molecular Interactions Involved in the Passage of the Cytotoxic Protein α-Sarcin Across Membranes....Pages 269-276
Oligosaccharyl Transferase and Protein Disulfide Isomerase: Two Key Enzymes in the Endoplasmic Reticulum....Pages 277-294
Division of the Golgi apparatus during mitosis in animal cells....Pages 295-303
Organelle Inheritance in Budding Yeast....Pages 305-311
Fusion Activity of Influenza Virus towards Target Membranes: pH Requirements and Effect of Dehydrating Agents....Pages 313-320
Structure and Regulation of a Gene Cluster Involved in Capsule Formation of Y.Pestis ....Pages 321-330
The Toxin of the Marine Alga Prymnesium Patelliferum Increases Cytosolic Ca 2+ in Synaptosomes and Voltage Sensitive Ca 2+ -Currents in Cultured Pituitary Cells....Pages 331-339
Semliki Forest Virus Induced Cell-Cell Fusion and Pore Formation....Pages 341-348
Back Matter....Pages 349-362

✦ Subjects

Biochemistry, general; Cell Biology