Biodistribution of monoclonal antibody A7 and its F(ab′)2 fragment in athymic nude mice bearing human pancreatic carcinoma

✍ Scribed by Eigo Otsuji; Toshiharu Yamaguchi; Nobuki Yamaoka; Nozomi Yamaguchi; Jiro Imanishi; Toshio Takahashi

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 502 KB
• Volume: 50
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930500309

✦ Synopsis

Xenografts of a human pancreatic carcinoma cell line, HPC-YS, which reacted with the monoclonal antibody (MAb) A7, were used to investigate the in vivo localization of radioiodinated MAb A7 after intraperitoneal injection. MAb A7 localized to the tumor 4 days and 8 days after injection with a tissue/blood ratio of 1.45 * 0.18 and 2.04 2 0.20, respectively.

The accumulation of MAb A7 in the tumor was 5%/g and 3.3%/g of the injected dose on day 4 and on day 8, respectively. In contrast, the F(ab'), fragment of MAb A7 localized to the tumor 4 days after intravenous injection with a tissue/blood ratio of 14.2. The accumulation of the F(ab'), fragment in the tumor was 1.2%/g of the injected dose. These results suggested that MAb A7 might be a suitable carrier of anticancer drugs for immunotargeting chemotherapy and that the F(ab'), fragment might be potentially useful for the immunodetection of human pancreatic carcinomas.