Biodistribution of a monoclonal antibody (RNL-1) against the neural cell adhesion molecule (NCAM) in athymic mice bearing human small-cell lung-cancer xenografts
✍ Scribed by O. C. Boerman; E. P. Mijnheere; J. L. V. Broers; G. P. Vooijs; F. C. S. Ramaekers
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- French
- Weight
- 795 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The purpose of this investigation was to determine the targeting potential of the murine monoclonal antibody (MAb) RNL-I for human small-cell lung cancer (SCLC) in a nude mouse model. RNL-I is preferentially reactive with SCLC and lung carcinoids, and was classified as a cluster-I MAb as defined by the International Workshop on Small-Cell Lung-Cancer Antigens. From the intercellular location of the target antigen and its reactivity with 3T3 cells transfected with nucleic acid sequences encoding for the neural cell adhesion molecule (NCAM), it was concluded that RNL-l is directed against NCAM. RNL-I was radiolabelled with either Iz5iodine or '"indium and injected into nude mice bearing NCI-HE2 SCLC xenografts. The biodistribution of the radiolabels was determined up to I20 hr post injection. Maximum tumour accretion for "'In-RNL-I was I1.8%ID/g and 6.5%ID/g for 1251-RNL-I. The accumulation of "'In-RNL-I could be visualized clearly by gamma scintigraphy without background subtraction techniques. Autoradiographs of whole-body sections from animals injected with '251-RNL-I showed that activity in the SCLC xenografts was mainly peripheral, suggesting that tumour uptake is dependent on the vascularization of the turnour tissue.